Ronald Robertson

Sanjay Singh

Scientist/Writer
  • Emailsanjaysingh765@gmail.com
  • Socail@lampatlex
  • VisitorSince 1982
  • LocationKentucky, USA

Blog


Topics



26Aug
How to rename fasta headers according to a matching name list

How to rename fasta headers according to a matching name list

FaBox  has several utilities to manipulate the FASTA sequence. I wanted to replace the FASTA header with the new header or description which...

28Feb
How to Extract Multiple Sequence from Multi Fasta File with PERL-III

How to Extract Multiple Sequence from Multi Fasta File with PERL-III

This is another PERL script to extract the FASTA sequence from a file by their accession number or ID stored in another file. 1. How to Extr...

26Feb
Sort FASTA File by Sequence Name

Sort FASTA File by Sequence Name

Problem I got a multi fasta file containing several isoform of the same locus. So my task was to get rid of the multiple version of same gen...

27Feb
Learn PERL Basic with Me : NCBI BLAST parser IV

Learn PERL Basic with Me : NCBI BLAST parser IV

Today's PERL script is very similar to the PERL script I shared yesterday . It will parse the NCBI blast output just like the previous o...

26Feb
Learn PERL Basic with Me : NCBI BLAST parser III

Learn PERL Basic with Me : NCBI BLAST parser III

I have already shared different parser to analyse the NCBI blast results. You can extract the best hits from your blast result file but thi...

13Feb
NCBI BLAST parser : Extract query and best hits II

NCBI BLAST parser : Extract query and best hits II

If you have been redirected to this post from Google or any search engine, you probably had entered “ NCBI BLAST parser ” or so in a despera...

31Jan
How to Extract Multiple Sequence from Multi Fasta File with PERL - II

How to Extract Multiple Sequence from Multi Fasta File with PERL - II

Previously I have shared a PERL script to extract multiple sequence from multi FASTA file with PERL . So if you have accession numbers store...

30Jan
How to Extract Longest Sequence Region Between Stop Codons in Translated DNA Sequences

How to Extract Longest Sequence Region Between Stop Codons in Translated DNA Sequences

So you have so many FASTA sequence in a file have translated those multiple nucleotide sequences and now you want to extract the region with...

24Jan
Extracting FASTA Sequences Based on Position WITH PERL Script

Extracting FASTA Sequences Based on Position WITH PERL Script

Previously, I have shared two different methods to extract fasta sequences based on position. For example, if you have coordinates for the n...

23Jan
How to Get Accession Numbers from FASTA File from GenBank

How to Get Accession Numbers from FASTA File from GenBank

Say if have retrieved multiple FASTA Sequences using Uniprot ID from Uniprot or NCBI and now you want to get the accession numbers from FA...

31Aug
How to Remove All Empty Fasta Sequences from a file using PERL script

How to Remove All Empty Fasta Sequences from a file using PERL script

Suppose a condition a where you have a file with multiple fasta file which contain several hundred FASTA sequence but few of them have only ...

09Jul
How to Split Multi line FASTA Files with PERL Script

How to Split Multi line FASTA Files with PERL Script

Recently someone anonymous (surprisingly most of the reader who comment on this blog are anonymous) asked for a PERL script to split a multi...

23May
How to BLAST multiple sequences against NCBI database using PERL script - II

How to BLAST multiple sequences against NCBI database using PERL script - II

Previously, I shared a PERL script to perform batch BLAST to the NCBI server . This PERL script uses Bioperl modules so make sure that biop...

21May
How to Add FASTA Description to Multiple Sequences

How to Add FASTA Description to Multiple Sequences

So you have a multi-fasta file like this GGCGGATGTAGCCACGTGGATCGGCGGATGTAGCCACGTGGATCGGCGGATGTAGCCACGTGGATCGGCGGATGTAGCCACGTGGATC AGCTGCTG...

05Apr
How to retrieve NCBI GenBank records with a range of accession numbers

How to retrieve NCBI GenBank records with a range of accession numbers

I have previously discussed the way to download the sequence from NCBI database if you have a list of accession numbers. But what if you h...

04Apr
How to Remove Duplicate Sequences from a multi fasta Files

How to Remove Duplicate Sequences from a multi fasta Files

Most common problems with sequence analysis is presence of duplicate sequence in data sets. Even protein or nucleotide sequences downloaded ...

12Mar
How to Extract FASTA Header With PERL Script

How to Extract FASTA Header With PERL Script

Previously I have already shared some tricks and PERL script to extract the FASTA header fro multi FASTA files. I am adding another PERL scr...

11Mar
How to Parse/Edit FASTA header

How to Parse/Edit FASTA header

Imagine a situation that you have thousands of FASTA sequences in a file and want to shorten or edit the FASTA header instead of whole long ...

24Jul
How to Extract Multiple Sequence from Multi Fasta File with PERL

How to Extract Multiple Sequence from Multi Fasta File with PERL

Yesterday, we talked about NCBI BLAST parsing . I shared two PERL script than can parsed BLAST results in tabulated manner so that you can a...

23Jul
NCBI BLAST parser : Extract query and best hits

NCBI BLAST parser : Extract query and best hits

BLAST is a wonderful utility for sequence analysis studies. Bioinformatic analysis of thousand of sequences without BLAST is unthinkable. Bu...