Ronald Robertson

Sanjay Singh

Scientist/Writer
  • Emailsanjaysingh765@gmail.com
  • Socail@lampatlex
  • VisitorSince 1982
  • LocationKentucky, USA

Blog


Topics



13Feb
NCBI BLAST parser : Extract query and best hits II

NCBI BLAST parser : Extract query and best hits II

If you have been redirected to this post from Google or any search engine, you probably had entered “ NCBI BLAST parser ” or so in a despera...

31Jan
How to Extract Multiple Sequence from Multi Fasta File with PERL - II

How to Extract Multiple Sequence from Multi Fasta File with PERL - II

Previously I have shared a PERL script to extract multiple sequence from multi FASTA file with PERL . So if you have accession numbers store...

30Jan
How to Extract Longest Sequence Region Between Stop Codons in Translated DNA Sequences

How to Extract Longest Sequence Region Between Stop Codons in Translated DNA Sequences

So you have so many FASTA sequence in a file have translated those multiple nucleotide sequences and now you want to extract the region with...

24Jan
Extracting FASTA Sequences Based on Position WITH PERL Script

Extracting FASTA Sequences Based on Position WITH PERL Script

Previously, I have shared two different methods to extract fasta sequences based on position. For example, if you have coordinates for the n...

23Jan
How to Get Accession Numbers from FASTA File from GenBank

How to Get Accession Numbers from FASTA File from GenBank

Say if have retrieved multiple FASTA Sequences using Uniprot ID from Uniprot or NCBI and now you want to get the accession numbers from FA...

10Jan
How to Retrieving Multiple FASTA Sequence using Uniprot ID

How to Retrieving Multiple FASTA Sequence using Uniprot ID

Previously, I have shared two different tools to retrieve multiple sequence from NCBI. One was used to retrieve the many sequence record fro...

18Dec
DNA to RNA to Protein Transcription and Translation Tool

DNA to RNA to Protein Transcription and Translation Tool

Converts DNA to RNA to protein DNA sequence RNA sequence Protein sequence

06Oct
Extract Part of a FASTA Sequences with Position

Extract Part of a FASTA Sequences with Position

Update: 5.29.2018 Bedtools are another nice tool to extract defined regions sequence from FASTA file. Install Bedtools on your Ubuntu machi...

09Jul
How to Split Multi line FASTA Files with PERL Script

How to Split Multi line FASTA Files with PERL Script

Recently someone anonymous (surprisingly most of the reader who comment on this blog are anonymous) asked for a PERL script to split a multi...

23May
How to BLAST multiple sequences against NCBI database using PERL script - II

How to BLAST multiple sequences against NCBI database using PERL script - II

Previously, I shared a PERL script to perform batch BLAST to the NCBI server . This PERL script uses Bioperl modules so make sure that biop...

21May
How to Add FASTA Description to Multiple Sequences

How to Add FASTA Description to Multiple Sequences

So you have a multi-fasta file like this GGCGGATGTAGCCACGTGGATCGGCGGATGTAGCCACGTGGATCGGCGGATGTAGCCACGTGGATCGGCGGATGTAGCCACGTGGATC AGCTGCTG...

04Apr
How to Remove Duplicate Sequences from a multi fasta Files

How to Remove Duplicate Sequences from a multi fasta Files

Most common problems with sequence analysis is presence of duplicate sequence in data sets. Even protein or nucleotide sequences downloaded ...

12Mar
How to Extract FASTA Header With PERL Script

How to Extract FASTA Header With PERL Script

Previously I have already shared some tricks and PERL script to extract the FASTA header fro multi FASTA files. I am adding another PERL scr...

11Mar
How to Parse/Edit FASTA header

How to Parse/Edit FASTA header

Imagine a situation that you have thousands of FASTA sequences in a file and want to shorten or edit the FASTA header instead of whole long ...

09Nov
How to Use New NCBI BLAST Result Interface

How to Use New NCBI BLAST Result Interface

On NCBI BLAST result page (right up corner, in red font), you might notice a notification ' View these results in the new enhanced repo...

24Jul
How to Extract Multiple Sequence from Multi Fasta File with PERL

How to Extract Multiple Sequence from Multi Fasta File with PERL

Yesterday, we talked about NCBI BLAST parsing . I shared two PERL script than can parsed BLAST results in tabulated manner so that you can a...

23Jul
NCBI BLAST parser : Extract query and best hits

NCBI BLAST parser : Extract query and best hits

BLAST is a wonderful utility for sequence analysis studies. Bioinformatic analysis of thousand of sequences without BLAST is unthinkable. Bu...

17Jul
How to Filter the Sequence by Their Length

How to Filter the Sequence by Their Length

PERL scripts are most wonderful stuff because they can help you in almost all aspect of Bioinformatics. PERL script can perform BLAST , par...